Journal: Nanoscale Advances

Article Title: Magnetic nanoparticle mediated-gene delivery for simpler and more effective transformation of Pichia pastoris †

doi: 10.1039/d1na00079a

Figure Lengend Snippet: Confocal microscopy demonstration of cellular uptake of MNPs in yeast treated with TRITC-labelled MNPs. (A) 2D confocal microscope images. The scale bar is 5 μm. (B) Quantitative analysis of confocal images. (C) Z -Stack images from yeast treated with TRITC-labelled MNPs.

Article Snippet: The size and morphology of the Fe 3 O 4 @PEI MNPs were obtained via transmission and scanning electron microscopy (TEM, Hitachi HighTech HT7700, and SEM, Carl Zeiss), respectively.

Techniques: Confocal Microscopy, Microscopy

Journal: Nanoscale Advances

Article Title: Magnetic nanoparticle mediated-gene delivery for simpler and more effective transformation of Pichia pastoris †

doi: 10.1039/d1na00079a

Figure Lengend Snippet: Integration of GFP coding sequence into the P. pastoris genome. Agarose gel electrophoresis image of PCR products performed with (A) the gene specific primers (GFP forward and GFP reverse; 731 bp) and (B) the vector specific primers (pGAP forward and 3′AOX1; 963 bp). Also, magneto-transformation was performed with pGKB plasmid (276 bp). (C) gDNA and gene specific or/gene and vector specific primers (pGAP forward and GFP reverse; 767 bp). M: marker and V: vector, pGKB-GFP. (D) The images of GFP produced colonies after magneto-transformation by confocal microscopy. The scale bar is 10 μm. (E) The fluorescence intensity result was calculated from confocal images.

Article Snippet: The size and morphology of the Fe 3 O 4 @PEI MNPs were obtained via transmission and scanning electron microscopy (TEM, Hitachi HighTech HT7700, and SEM, Carl Zeiss), respectively.

Techniques: Sequencing, Agarose Gel Electrophoresis, Plasmid Preparation, Transformation Assay, Marker, Produced, Confocal Microscopy, Fluorescence